Expression Microarray

mRNA Expression Microarray

mRNA (messenger RNA) is a type of RNA produced when DNA goes through transcription. mRNA includes the coding region that contains protein information. Analysis of mRNA’s expression pattern is crucial to finding the gene that carries out the main function in a certain environment and the gene’s pathway.

With an mRNA expression microarray, tests are performed for the mRNA analysis of various organisms and significant genes can be selected by conducting a statistical analysis of the data obtained from the tests.

Expression Microarray Platforms

Manufacturer Specimen Platform
Agilent - Human
- Mouse
- Rat
- Model Org/Non-Human
SurePrint G3 Gene Expression
SurePrint G3 Exon
Customization
Others
Affymetrix Gene ST (Whole Transcript)
GenomeArray (3'IVT)
Clariom™
Others
Life Technologies Taqman mRNA Expression
Openarray Customization

Analysis

Analysis Items Contents
Data analysis of DE Basic statistics (fold change, group mean, SD, etc.)
Identifying differentially expressed genes (T-test, LPE test, ANOVA, etc.)
Multiple testing correction (FDR, Bonferroni, etc.)
Clustering Analysis for DEG (hierarchical clustering, K-means, etc.)
Functional analysis KEGG pathway, GeneOntologyannotation (DAVID, goProfiles, GOstats, etc.)
GeneSet Enrichment Analysis
Customized analysis Integrative analysis (mRNA-miRNA, methylation~mRNA, mRNA-CNV, etc.)

microRNA Expression Microarray

microRNA is a single-sequence small RNA composed of 21 – 25 nucleotides. microRNA controls gene expression by interrupting mRNA’s function of forming protein via the translation process. The microRNA study can widen the understanding of RNA silencing.

microRNAs exist in many forms according to the generation process. We have many microarray products to analyze the expression patterns of mature miRNAs known by studies. Macrogen provides analysis services including selection of microRNAs where differential expression is confirmed and target mRNA prediction, as well as test services using microarrays.

miRNA Expression Microarray Platforms

Manufacturer Specimen Platform
Agilent - Human
- Mouse
- Rat
- Model Org/Non-Human
SurePrint G3 miRNA Expression
Customization
Affymetrix miRNA 4.0 (203 specimens)
Life Technologies Taqman miRNA Expression

Analysis

Analysis Items Contents
Data analysis of DE Basic statistics (fold change, group mean, SD, etc.)
Identifying differentially expressed genes (T-test, LPE test, ANOVA etc.)
Multiple testing correction (FDR, Bonferroni, etc.)
Clustering Analysis for DEG (hierarchical clustering, K-means, etc.)
Customized analysis Target gene prediction
Integrative analysis (mRNA-miRNA)