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Meaningful genes can be discovered
through the followings:
 Analysis of the intensity quantity and gene image per spot through Axon’s GenePix software
Normalization process wherein systematic variations affecting gene expression are detected and
removed
Statistical techniques such as Fold change, Clustering, Classification, PCA, T-test,
Permutation test, and ANOVA
Functional gene classification can also be confirmed through the GO (Gene Ontology) Pathway. |
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Analyzing the signal intensity
of the spot using GenePix 5.1 software after image scanning
using an Axon scanner |
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Removing the systematic variations affecting gene expression |
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Global normalization |
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Intensity normalization |
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print-tip normalization |
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Quantile normalization |
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Scale normalization |
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Multiple slide normalization |
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MA plot |
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Scatter plot |
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Normalization Density |
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Grouping according to gene expression
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Analysis
using various algorithms, filters,
and options |
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Hierarchical |
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K-means |
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SOM |
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Test process to find meaningful genes
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Fold Change |
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Classification |
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T-test (F-test) |
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ANOVA |
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Multiple testing |
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FDR |
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Permutation test |
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SAM(Significance Analysis of
Microarray) |
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Gene Bank Accession Number |
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Cluster ID (Unigene site와 hyperlink) |
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Title (Unigene site와 hyperlink
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Gene Ontology (Functional grouping) |
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| Sends analysis results within 10 days of sample receipt |
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Data on sample quality check |
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Data description |
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Summary of analysis results |
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Normalized data |
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Scatter plot |
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Gene annotation information (periodical update) |
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Functional grouping (gene ontology) |
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Customized analysis upon the client’s request |
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