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How long does Knockout mouse production take? |
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Going through the entire process of producing a knockout mouse takes at least 12 months, although the production period may be shortened or extended depending on the gene location, type, and other factors. |
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How much does a Knockout mouse cost? |
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Total costs amount to KRW 28,800,000, although the price may vary according to the number of screening samples and production method. For details, make a separate price inquiry. |
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There are various processes involved in Knockout mouse production. Is a separate service for each process available?
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Yes. Before the relevant process is conducted, however, accurate and clear verification of products (KO vector, positive ES clone) is executed. |
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Where can I get information on a target gene? How much information do we need to have? |
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In the case of known gene functions, you can get the necessary information (genome sequence, mRNA sequence, etc.) through websites such as NCBI and Ensemble. In addition, Macrogen receives and processes all orders through mouse gene information on hand to enhance the security of the experimental genes requested by the client. |
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What kind of ES cell is used? |
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We use the ES cell of the 129/SvJ strain, although we plan to offer services using the ES cell of the C57BL strain in the near future. |
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What kind of screening method is used? |
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We provide screening services using PCR and Southern blot.
For Southern blot in particular, the non-radioactive method is used. |
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Do you offer conditional knockout mouse production service? |
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No, but we plan to offer such service in the immediate future. |
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