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Construction of the targeting vector (when requesting for vector design): |
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Data on cDNA, mRNA, and genome sequence of a target gene |
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Accurate information and sequence concerning genes: What kind of gene? |
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What is the source of the gene? (e.g., mouse gene, human gene, etc.) |
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Purification of expression vector
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Completed vector: Prepare 10ug or more of Plasmid DNA with concentration of 100ng/ul or more. |
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Electroporation and Screening : |
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Completed KO vector: Detailed vector information (Southern blot information, linearization information, genome sequence, etc.) |
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Screening-related Southern probe production and specific verification as well as PCR primer services can be provided by Macrogen at affordable prices. |
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Identification of germline transmission
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Strain, source, and other information on ES clone provided |
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