 |
|
 |
What kind of mouse strain is used for TG production? |
| |
C57BL/6 (black mouse) is generally used, although any client request for a specific kind of strain (FVB/n, BALB/c, etc.) is adopted upon consultation. |
|
|
When making a vector, what should the client do? |
| |
There is no separate vector for TG production. The client may use an appropriate vector for cloning and check whether the desired gene was expressed after production. |
|
|
How should the client construct the vector to express the desired gene efficiently? |
| |
The
gene used for constructing a vector, i.e., cDNA, genomic
DNA, or BAC DNA (production for BAC TG) is not a factor.
To express the gene efficiently, poly A is attached to
3' with the mini gene construct where some of the intron
or genomic DNA are inserted between cDNAs |
|
|
How large is the gene that can be used for TG production? |
| |
Based on know-how accumulated through the years, Macrogen faces no problem producing TG using a large gene (10kb or more). In addition, it has commercialized BAC TG mice with size of 200kb or more, such as BAC (Bacterial Artificial Chromosome) DNA. |
|
|
Upon the completion of a vector, how is the gene prepared? |
| |
The completed vector is prepared under Plasmid DNA conditions of 20ug or more. Together with Plasmid DNA, the restriction site of the completed vector and the map where the vector backbone is indicated are furnished. |
|
|
How long does it take to produce TGs? How many TGs are produced? How much do they cost? |
| |
If you send a completed vector, it takes about 3 months (the period may vary per gene) to produce 3 founder TG mice, which cost KRW 5,600,000. Requests for vector construction require longer production period and incur additional costs. |
|
|
Is a separate service for each process available? |
| |
Yes. To provide customized quality services, Macrogen also offers services for specific processes preferred by the customer throughout the entire transgenic mouse production process. |
|
|
What kind of method is used to screen transgenic mice? |
| |
To screen transgenic mice, Macrogen extracts genomic DNA from a mouse’s tail and carries out PCR screening using a specific primer. Basically, PCR screening is performed, although screening using Southern blot may also be executed upon the request of the client. |
|
