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DNA sources
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Formats |
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- Mammalian
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- Standard cDNA library |
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- Plant
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- Average Insert size :
1.5~2.5kb |
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- Insert size range : 0.5~4.5kb |
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Time
required
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- Total number of primary
clones : 1.0X107 |
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- 4~6 weeks |
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| Step 1 : |
Total RNA check |
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| Step 2 :
| Synthesize cDNA |
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Synthesizes first-strand cDNA through the
Oligo (dT) linker-primer and reverse transcriptase;
synthesizes
second-strand cDNA using DNA polymerase I. Ligates an
appropriate adaptor.
* Optional: normalization (Removes cDNA of higher frequency) |
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| Step 3 : |
Size selection
and cloning |
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Ligates to an appropriate vector after
size fraction through gel filtration. |
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| Step 4 : |
Analysis |
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- Redundancy check
- Blast service
- GO (gene ontology report)
- Others |
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Library report |
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Average insert size check |
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Insert size range check |
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Test analysis : Redundancy check and blast
service |
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Provision of library |
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Provision of phagemid or cell or DNA |
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| Analysis report |
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Redundancy check |
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Blast service |
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GO (gene ontology report) |
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Others |
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