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DNA Sources
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Library Sources |
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- Mammalian
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- Insert Preparation : Random
Shearing |
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- Plant
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- Insert Size : 35~45 kb
(Insert Size Std : 5kb< ) |
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- Bacteria
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- Library format : Cell or
DNA |
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Time
Required |
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- 4~5 weeks after checking
whether each sample is normal |
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| Step 1 : |
DNA preparation |
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Separates High Molecular Weight DNA (100~400kb) from
the host. |
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| Step 2 :
| Insert Preparation
1 |
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Random Shearing: Secures the insert with
the desired size through size selection in Agarose gel
after random shearing using a nebulizer. |
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| Step 3 : |
Insert Preparation
2 |
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Processes the insert to be ligated to the
vector appropriately. |
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| Step 4 : |
Ligation |
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After ligation to Cosmid Vector, packages
the recombinant cosmid DNA into bacteriophage lamda particles
using Lamda Packaging Extract in vitro and constructs
a library by transducing them to an appropriate host. |
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Library Report |
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Insert Size (Std 1kb<) |
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Insert Ratio (95%>) |
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TEST Sequencing Result |
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Provision of Library |
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Provision of Cell or DNA |
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