-
10~15ul with concentration of 100ng/ul based
on Plasmid
- 10~15ul with concentration of 50ng/ul based
on purified PCR product
(20-30ul in case of unpurified
PCR product)
- 1.5mL microcentrifuge tube recommended |
|
-
10ul with concentration of 5 pmole/ul (5uM)
based on 5 samples
- Sufficient quantity required (increase of
10 pmole each time the reactions are increased)
- Vessel separated from samples at the time
of provision of the client’s primer
- Primer Tm suitable for Macrogen service:
50~58 ℃ |
|
-
Sequencing conducted under BigDyeTM terminator
cycling conditions
- The reacted products purified using Ethanol
Precipitation and run using Automatic
Sequencer 3730xl |
|
-
Phred score of 16 or higher and read length
of 500bp or more guaranteed based on the
normal plasmid template
- Abi file (electropherogram, raw data) created
after 3730xl completes analysis
- Further detailed analysis conducted using
the Macrogen analysis module; the results
together with the raw data are
uploaded to the client’s account
- Notification mail automatically sent upon
the completion of analysis; client may then
access LIMS and download the
results
- Possible to use the Bioinformatics Service
separately |
