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Home > Services > Whole Genome Service > Shotgun library construction

	DNA sources			Library sources
	- Mammalian			- Insert preparation : Random shearing or partial digestion
	- Plant			- Insert size : 1~8kb Insert plasmid library (Insert size : 2~5kb)
	- Bacteria			- Vector : High copy number vector or low copy number vector
	- Virus			- Library format : Cell or DNA
	- Bacteriophage
	- BAC DNA			Time Required
	- Cosmid DNA			-check whether sample confirm after 4 weeks.
	- Fosmid DNA

Step 1 :	DNA preparation
	a. b.	Genomic shotgun library: Separates high molecular weight DNA (100~400kb) from the host. Large costruct shotgun library (BAC, Cosmid, Fosmid, Plasmid, etc.): Separates the sample DNA from the host and removes the host genomic DNA. Checks the samples based on the size of the prepared sample DNA and the results of end sequencing.

Step 2 :	Insert preparation 1
	a.	Random shearing method: Secures the insert with the desired size by size-selecting DNA(2~5kb) obtained through hydroshear random shearing using agarose gel electrophoresis once again

Step 3 :	Insert preparation 2
	Processes the insert to be ligated to the vector appropriately (blunting or CIP process).

Step 4 :	Ligation
	Checks the quality of the library by ligating it to high copy vector or low copy vector.