 |
| |
The
genome base sequence analysis of Macrogen
is
largely classified into 3 parts:
Part I
- 2~5kb random shotgun library
construction
- 10kb insert plasmid library (Random sheared insert
or partially digested insert)
- Sequencing through DNA Analyzer 3730xl
- Assembly using ARACHNE
- Gap filling |
| |
Part
II
| - Gene
annotation (characterization of the
predicted
ORFS and prediction of structural RNA) |
| |
a.
|
BlastX
Non redundant protein databases |
| |
b.
|
Cluster
of Orthologous Group (NCBI) |
| |
c.
|
pFAM Protein
families database of alignments and HMMs |
| |
d.
|
tRNA scan
SE tRNA finding program (WashU) |
|
| |
Part
III
| - |
Provision of genome
browser (Genome browser is implemented
to
enable the client to check |
| |
the complete
results of the experiment and customization
according
to the client’s request) |
|
