Spatial Multi-Omics Analysis

Macrogen provides personalized single cell multi-omics analysis services based on extensive experience and innovation.

Single-cell gene sequencing is used to analyze gene expression profile and genetic changes in a single cell, which is a functional and structural unit of an organism. As it becomes possible to determine cellular heterogeneity, rare cell population, cellular differentiation, chromatin heterogeneity, immune profile, and surface protein profile in a single cell unit, this technique is applied to various fields of research including selection of cell treatments, stem cells, and cancer development and progression. Macrogen quickly provides a high level of genetic sequencing data based on its ability to perform NGS (next generation sequencing), which ranks first in Korea and fifth in the world. Furthermore, we are leading the market by diversifying single-cell sequencing services, thanks to industry-leading genome sequencing expertise accumulated over 26 years and investment in big data and unexplored areas.

Spatial Multi-Omics Analysis 소개이미지

마크로젠은 10x Genomics와 Bruker 등 글로벌 선도 기업들의 최신 플랫폼(Visium, Xenium, CosMX, GeoMX 등)을 활용하여 공간 다중오믹스 서비스를 제공합니다. 특히 국내 1위이자 세계 5위 수준의 차세대염기서열분석(Next Generation Sequencing, NGS) 역량과 더불어, 최신 in situ 기반 공간 분석 기술까지 도입하여, 연구 목적에 최적화된 고품질 데이터를 빠른 시간 내에 제공합니다. 마크로젠은 기술 도입과 서비스 확장을 선도함으로써 공간 다중오믹스 분석 분야에서 연구자들에게 최적의 파트너가 되고 있습니다.

Spatial Multi-Omics Analysis
NGS-based Spatial Omics
  • Visium (10x Genomics)
  • GeoMx® DSP (Bruker)
In situ-based Spatial Omics
  • Xenium (10x Genomics)
  • CosMx ® SMI (Bruker)

PLATFORM

NGS-based Spatial omics

In situ-based Spatial omics

Visium Spatial

Uncover the whole transcriptome with spatial context

Visium : Sample Preparation

FF
(Fresh Frozen)
FFPE
(Formalin Fixed Paraffin Embedded)
Sectioned slide
Sample type OCT (compound) block Paraffin block FF / FFPE
Section Thickness 10 μm 5 μm FF 10µm / FFPE 5µm
RNA Quality RIN ≥ 7 DV200 ≥ 50% FF RIN ≥ 4 / FFPE DV200 ≥ 30%
Species Any organism (poly A tail) Only Human, Mouse Only Human, Mouse
Quality Check Process
  • Visium-FF : Section (Cryotome) - RNA QC & ROI* check - Tissue optimization test
  • Visium-FFPE : Section (Microtome) - RNA QC & ROI check - Tissue adhesion test
  • Visium CytAssist : RNA QC & ROI check - no tissue optimization, tissue adhesion test

*ROI : Region of interest

GeoMx® DSP

Discover Where Biology Happens

Platform GeoMx® Digital Spatial Profiler
Sample FFPE Block (Storage period: 3 years / 35.3mm x 14.1 mm x 4-6 µm / Core Needle Biopsy available)
Fresh Frozen Tissue (Store at -80℃, in cutting, within 6 months/ 35.3mm x 14.1 mm x 4-6 µm )
Panel Human Whole Transcriptome Atlas (hWTA)

Xenium In Situ

High-plex in situ from the single cell leader

Platform Xenium In Situ Analyzer
TAT 6 Weeks
Sample type FFPE, Fresh Frozen Tissue
RNA & Protein Assay Human Breast Gene Expression Panel (280 genes + Customize up to 100 genes)
Extensive panel roadmap (Human Brain, Lung, Skin, Kidney, Pancreas)
Various RNA targets analysis can be provided upon request
Xenium in-situ gene expression panels
Prime 5K Human pan tissue and pathways (5001 genes) Add on
up to 100
genes
Mouse pan tissue and pathways (5006 genes)
Xenium v1 Human immuno-oncology (380 genes)
Human breast (280 genes)
Human lung (289 genes)
Human brain (266 genes)
Human multi-tissue & cancer (377 genes)
Human colon (322 genes)
Human skin (260 genes)
Mouse brain (248 genes)
Mouse multi-tissue (379 genes)

CosMx™ SMI

The First Fully-Integrated Single-Cell Spatial Biology Solution with the highest plex, multiomic capability and high resolution

Platform CosMx™ Spatial Molecular Imager
TAT 5 Weeks after ROI section
Sample type FFPE Block (Storage period: 3 years / 35.3mm x 14.1 mm x 4-6 µm / Core Needle Biopsy available)
Fresh Frozen Tissue (Store at -80℃, in cutting, within 6 months / 35.3mm x 14.1 mm x 4-6 µm )
tissue microarray, organoid sample
RNA & Protein Assay CosMx™ Human Universal Cell Characterization Panel (RNA, 1000 Plex)
CosMx™ Human Immuno-oncology Panel (RNA, 100 Plex)
CosMx™ Human Immuno-oncology Panel (Protein, 64 Plex)
Please contact us for inquiries about other panel assays
We offer simultaneous analysis for RNA and protein from sectioned tissue.
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